Chapter title |
Control of Pro-inflammatory Cytokine Release from Human Monocytes with the Use of an Interleukin-10 Monoclonal Antibody.
|
---|---|
Chapter number | 8 |
Book title |
Cytokine Bioassays
|
Published in |
Methods in molecular biology, January 2014
|
DOI | 10.1007/978-1-4939-0928-5_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-0927-8, 978-1-4939-0928-5
|
Authors |
Hardik Patel, Dennis Davidson, Patel H, Davidson D |
Abstract |
The monocytes (MONOs) can be considered as "double-edge swords"; they have both important pro-inflammatory and anti-inflammatory functions manifested in part by cytokine production and release. Although MONOs are circulating cells, they are the major precursors of a variety of tissue-specific immune cells such as the alveolar macrophage, dendritic cells, microglial cells, and Kupffer cells. Unlike the polymorphonuclear leukocyte, which produces no or very little interleukin-10 (IL-10), the monocyte can produce this potent anti-inflammatory cytokine to control inflammation. IL-10, on an equimolar basis, is a more potent inhibitor of pro-inflammatory cytokines produced by monocytes than many anti-inflammatory glucocorticoids which are used clinically. This chapter describes how to isolate monocytes from human blood and the use of IL-10 monoclonal antibody to determine the effect and timing of endogenous IL-10 release on the production and release of pro-inflammatory cytokines. |
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