Chapter title |
Small-Molecule High-Throughput Screening Utilizing Xenopus Egg Extract.
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Chapter number | 5 |
Book title |
Chemical Biology
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Published in |
Methods in molecular biology, January 2015
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DOI | 10.1007/978-1-4939-2269-7_5 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2268-0, 978-1-4939-2269-7
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Authors |
Matthew R Broadus, P Renee Yew, Stephen R Hann, Ethan Lee, Broadus MR, Yew PR, Hann SR, Lee E, Matthew R. Broadus, P. Renee Yew, Stephen R. Hann, Broadus, Matthew R., Yew, P. Renee, Hann, Stephen R., Lee, Ethan |
Abstract |
Screens for small-molecule modulators of biological pathways typically utilize cultured cell lines, purified proteins, or, recently, model organisms (e.g., zebrafish, Drosophila, C. elegans). Herein, we describe a method for using Xenopus laevis egg extract, a biologically active and highly tractable cell-free system that recapitulates a legion of complex chemical reactions found in intact cells. Specifically, we focus on the use of a luciferase-based fusion system to identify small-molecule modulators that affect protein turnover. |
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Unknown | 1 | 100% |
Demographic breakdown
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Student > Ph. D. Student | 1 | 100% |
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Agricultural and Biological Sciences | 1 | 100% |