Chapter title |
High Content Screening for Modulators of Cardiac Differentiation in Human Pluripotent Stem Cells.
|
---|---|
Chapter number | 4 |
Book title |
Chemical Biology
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2269-7_4 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2268-0, 978-1-4939-2269-7
|
Authors |
Sean Spiering, Herman Davidovics, Paul J Bushway, Mark Mercola, Erik Willems, Paul J. Bushway, Spiering, Sean, Davidovics, Herman, Bushway, Paul J., Mercola, Mark, Willems, Erik |
Abstract |
Chemical genomics has the unique potential to expose novel mechanisms of complex cellular biology through screening of small molecules in in vitro assays of a biological phenotype of interest, followed by target identification. In the case of disease-specific assays, the cellular proteins identified might constitute novel drug targets, and the small molecules themselves might be developed as drug leads. In cardiovascular biology, a chemical genomics approach to study the formation of cardiomyocyte, vascular endothelial, and smooth muscle lineages might contribute to therapeutic regeneration. Here, we describe methods used to develop high content screening assays implementing multipotent cardiovascular progenitors derived from human pluripotent stem cells and have identified novel compounds that direct cardiac differentiation. |
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Unknown | 1 | 100% |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
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Unknown | 15 | 100% |
Demographic breakdown
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Student > Ph. D. Student | 4 | 27% |
Researcher | 3 | 20% |
Student > Bachelor | 3 | 20% |
Student > Master | 2 | 13% |
Other | 1 | 7% |
Other | 1 | 7% |
Unknown | 1 | 7% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 6 | 40% |
Medicine and Dentistry | 3 | 20% |
Agricultural and Biological Sciences | 3 | 20% |
Business, Management and Accounting | 1 | 7% |
Unknown | 2 | 13% |