Chapter title |
Fission Yeast-Based High-Throughput Screens for PKA Pathway Inhibitors and Activators.
|
---|---|
Chapter number | 6 |
Book title |
Chemical Biology
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2269-7_6 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2268-0, 978-1-4939-2269-7
|
Authors |
Ana Santos de Medeiros, Grace Kwak, Jordan Vanderhooft, Sam Rivera, Rachel Gottlieb, Charles S Hoffman, Charles S. Hoffman |
Abstract |
Features of the fission yeast Schizosaccharomyces pombe cAMP/PKA pathway make S. pombe particularly amenable for heterologous expression of cAMP pathway proteins such as GαS subunits and their cognate adenylyl cyclases, PKA catalytic and regulatory subunits, and cyclic nucleotide phosphodiesterases. We have constructed two PKA-repressed reporters for use in high-throughput screens to detect compounds that elevate or reduce PKA activity, thus facilitating the discovery of both inhibitors and activators of these target proteins. Here, we describe steps to construct screening strains and to optimize and conduct these screens. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 6 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 3 | 50% |
Student > Bachelor | 1 | 17% |
Unknown | 2 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 33% |
Agricultural and Biological Sciences | 2 | 33% |
Unknown | 2 | 33% |