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Mendeley readers
Chapter title |
In Vitro Analysis of Tem1 GTPase Activity and Regulation by the Bfa1/Bub2 GAP
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Chapter number | 6 |
Book title |
The Mitotic Exit Network
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Published in |
Methods in molecular biology, January 2017
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DOI | 10.1007/978-1-4939-6502-1_6 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6500-7, 978-1-4939-6502-1
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Authors |
Marco Geymonat, Adonis Spanos, Katrin Rittinger, Geymonat, Marco, Spanos, Adonis, Rittinger, Katrin |
Abstract |
Tem1 is a small GTPase that controls the mitotic progression of Saccharomyces cerevisiae through the Mitotic Exit Network. Tem1 activity is tightly controlled in mitosis by Bub2 and Bfa1 and is also regulated by the spindle orientation checkpoint that monitors the correct alignment of the mitotic spindle with the mother-daughter axis. In this chapter we describe the purification of Tem1, Bfa1, and Bub2 and a detailed radioactive filter-binding assay to study the nucleotide binding properties of Tem1 and the role of its regulators Bfa1 and Bub2. |
Mendeley readers
The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 4 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Professor > Associate Professor | 1 | 25% |
Student > Bachelor | 1 | 25% |
Researcher | 1 | 25% |
Student > Doctoral Student | 1 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 50% |
Agricultural and Biological Sciences | 1 | 25% |
Medicine and Dentistry | 1 | 25% |