| Chapter title |
Cdc14 and Chromosome Condensation: Evaluation of the Recruitment of Condensin to Genomic Regions
|
|---|---|
| Chapter number | 17 |
| Book title |
The Mitotic Exit Network
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6502-1_17 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6500-7, 978-1-4939-6502-1
|
| Authors |
Facundo Ramos, Joanne Leonard, Andrés Clemente-Blanco, Luis Aragón, Ramos, Facundo, Leonard, Joanne, Clemente-Blanco, Andrés, Aragón, Luis |
| Abstract |
Chromosome condensation is an essential morphological event required for successful DNA segregation during mitosis. The high level of genome compaction achieved during this process is attained by the evolutionary conserved condensin complex. Recently, several lines of evidences have demonstrated that the mitotic phosphatase Cdc14 is required to ensure condensin loading onto chromosomes. To date several approaches have been used in order to characterize condensin activity and regulation, however these techniques are time-consuming and require complex equipment. In this chapter we described an easy and reliable protocol to analyze Cdc14-dependent condensin loading onto specific genomic DNA regions by using a chromatin immunoprecipitation (ChIP) technique. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 8 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 2 | 25% |
| Professor | 1 | 13% |
| Student > Bachelor | 1 | 13% |
| Student > Master | 1 | 13% |
| Researcher | 1 | 13% |
| Other | 0 | 0% |
| Unknown | 2 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 3 | 38% |
| Agricultural and Biological Sciences | 2 | 25% |
| Environmental Science | 1 | 13% |
| Unknown | 2 | 25% |