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Mendeley readers
Chapter title |
Evaluation of the Nucleolar Localization of the RENT Complex to Ribosomal DNA by Chromatin Immunoprecipitation Assays
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Chapter number | 15 |
Book title |
The Mitotic Exit Network
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Published in |
Methods in molecular biology, January 2017
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DOI | 10.1007/978-1-4939-6502-1_15 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6500-7, 978-1-4939-6502-1
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Authors |
Julie Huang, Nahid Iglesias, Danesh Moazed, Huang, Julie, Iglesias, Nahid, Moazed, Danesh |
Abstract |
Chromatin immunoprecipitation (ChIP) is a valuable technique for localizing proteins of interest to specific genomic sites and determining the relative abundance of these proteins at these sites. The ChIP method entails chemical cross-linking of proteins to genomic DNA, isolation of protein-DNA conjugates, and purification of DNA from conjugates. Real-time polymerase chain reactions are used to identify and quantify isolated genomic sequences. Here we describe how to localize yeast proteins to gene sequences residing within the nucleolus, i.e., ribosomal DNA (rDNA). |
Mendeley readers
The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Spain | 1 | 25% |
Unknown | 3 | 75% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 3 | 75% |
Student > Doctoral Student | 1 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 3 | 75% |
Biochemistry, Genetics and Molecular Biology | 1 | 25% |