Chapter title |
A New Methodology for the Quantification of In Vivo Cdc14 Phosphatase Activity
|
---|---|
Chapter number | 8 |
Book title |
The Mitotic Exit Network
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6502-1_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6500-7, 978-1-4939-6502-1
|
Authors |
Ethel Queralt, José-Antonio Rodriguez-Rodriguez, Queralt, Ethel, Rodriguez-Rodriguez, José-Antonio |
Abstract |
The phosphatase Cdc14 has a pivotal function in the mitotic exit of Saccharomyces cerevisiae. During interphase, Cdc14 remains inactive in the nucleolus bound to the inhibitor Net1. Cdc14 activation occurs in the metaphase to anaphase transition and it is promoted by at least two signaling pathways called FEAR (CdcFourteen Early Anaphase Release) and MEN (Mitotic Exit Network). These two pathways act in parallel and target the phosphorylation of Net1, thus decreasing Net1 affinity for Cdc14. The activity of Cdc14 can be used as a readout to assay functional interactions of different components of the mitotic exit signaling pathways. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 5 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 2 | 40% |
Student > Bachelor | 1 | 20% |
Student > Ph. D. Student | 1 | 20% |
Student > Postgraduate | 1 | 20% |
Readers by discipline | Count | As % |
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