Chapter title |
Human Induced Pluripotent Stem Cell-Derived Sensory Neurons for Fate Commitment of Bone Marrow Stromal Cell-Derived Schwann Cells
|
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Chapter number | 10 |
Book title |
Schwann Cells
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7649-2_10 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7648-5, 978-1-4939-7649-2
|
Authors |
Sa Cai, Daisy K. Y. Shum, Ying-Shing Chan, Cai, Sa, Shum, Daisy K. Y., Chan, Ying-Shing |
Abstract |
Here we describe the in vitro derivation of sensory neurons for use in effecting fate commitment of Schwann cell-like cells derived from human bone marrow stromal cells (hBMSCs). We adopt a novel combination of small molecules in an 8-day program that induces the differentiation of human induced pluripotent stem cells into sensory neurons. In co-cultures, the derived sensory neurons present contact-dependent cues to direct hBMSC-derived Schwann cell-like cells toward the Schwann cell fate. These derived human Schwann cells survive passaging and cryopreservation, retain marker expression despite withdrawal of glia-inducing medium and neuronal cues, demonstrate capacity for myelination, and therefore promise application in autologous transplantation and re-myelination therapy. |
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