Chapter title |
A Rat Burn Injury Model for Studying Changes in Microvascular Permeability
|
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Chapter number | 8 |
Book title |
Traumatic and Ischemic Injury
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7526-6_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7524-2, 978-1-4939-7526-6
|
Authors |
Katie Wiggins-Dohlvik, Binu Tharakan |
Abstract |
The management of burn patients is an extremely complex and clinically challenging for patient care. Aside from the increasing reports of burn injury and morbidity and mortality directly related to it, the pathobiology of burn trauma is not clearly understood. The rat model of burn trauma described here is currently used in research laboratories to study various aspects of burn injury, including vascular dysfunctions. This model demonstrates the infliction of thermal injury in Sprague-Dawley rats using a well-established boiled water approach. We have utilized intravital microscopy to examine the microvascular hyperpermeability, the excessive leakage of proteins and fluids from the intravascular space to the extravascular space in mesenteric postcapillary venules using this model. An increase in microvascular permeability is a strong indicator of microvascular dysfunctions leading to tissue edema in burn trauma. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 5 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Professor | 1 | 20% |
Student > Bachelor | 1 | 20% |
Other | 1 | 20% |
Lecturer > Senior Lecturer | 1 | 20% |
Unknown | 1 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 3 | 60% |
Nursing and Health Professions | 1 | 20% |
Unknown | 1 | 20% |