| Chapter title |
Methods to Study the Innate Immune Response to Sepsis
|
|---|---|
| Chapter number | 15 |
| Book title |
Traumatic and Ischemic Injury
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7526-6_15 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7524-2, 978-1-4939-7526-6
|
| Authors |
Wendy E. Walker |
| Abstract |
This chapter describes techniques to measure the innate immune response in the mouse cecal ligation and puncture model of sepsis. The reader will learn how to perform retro-orbital bleeds to harvest serum from mice and learn how to perform peritoneal lavage to harvest cells and inflammatory mediators from this compartment. The enzyme-linked immunosorbent assay (ELISA) technique is described as a method to measure the levels of cytokines and chemokines in these fluids. Additionally, this chapter describes techniques to stain the cellular fraction of the peritoneal lavage with fluorescently labeled antibodies, and perform fluorescence activated cell sorting (FACS) to quantify macrophages and neutrophils in this compartment. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 11 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 2 | 18% |
| Student > Ph. D. Student | 1 | 9% |
| Professor > Associate Professor | 1 | 9% |
| Student > Master | 1 | 9% |
| Unknown | 6 | 55% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 2 | 18% |
| Immunology and Microbiology | 2 | 18% |
| Neuroscience | 1 | 9% |
| Medicine and Dentistry | 1 | 9% |
| Unknown | 5 | 45% |