Chapter title |
Viral Metagenomics Approaches for High-Resolution Screening of Multiplexed Arthropod and Plant Viral Communities
|
---|---|
Chapter number | 7 |
Book title |
Viral Metagenomics
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7683-6_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7682-9, 978-1-4939-7683-6
|
Authors |
Sarah François, Denis Filloux, Emmanuel Fernandez, Mylène Ogliastro, Philippe Roumagnac, François, Sarah, Filloux, Denis, Fernandez, Emmanuel, Ogliastro, Mylène, Roumagnac, Philippe |
Abstract |
Viral metagenomic approaches have become essential for culture-independent and sequence-independent viral detection and characterization. This chapter describes an accurate and efficient approach to (1) concentrate viral particles from arthropods and plants, (2) remove contaminating non-encapsidated nucleic acids, (3) extract and amplify both viral DNA and RNA, and (4) analyze high-throughput sequencing (HTS) data by bioinformatics. Using this approach, up to 96 arthropod or plant samples can be multiplexed in a single HTS library. |
X Demographics
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Unknown | 1 | 100% |
Demographic breakdown
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 60 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Master | 12 | 20% |
Researcher | 11 | 18% |
Student > Ph. D. Student | 9 | 15% |
Student > Bachelor | 3 | 5% |
Student > Doctoral Student | 2 | 3% |
Other | 5 | 8% |
Unknown | 18 | 30% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 22 | 37% |
Biochemistry, Genetics and Molecular Biology | 7 | 12% |
Veterinary Science and Veterinary Medicine | 2 | 3% |
Immunology and Microbiology | 2 | 3% |
Medicine and Dentistry | 2 | 3% |
Other | 6 | 10% |
Unknown | 19 | 32% |