Chapter title |
Small RNA Isolation from Tissues of Grapevine and Woody Plants
|
---|---|
Chapter number | 2 |
Book title |
Viral Metagenomics
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7683-6_2 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7682-9, 978-1-4939-7683-6
|
Authors |
Annalisa Giampetruzzi, Michela Chiumenti, Angelantonio Minafra, Pasquale Saldarelli, Giampetruzzi, Annalisa, Chiumenti, Michela, Minafra, Angelantonio, Saldarelli, Pasquale |
Abstract |
A protocol is described to purify small (s)RNA molecules from tissues of grapevine and other woody plants. The protocol has been specifically developed to analyze sRNA populations by high-throughput sequencing. It has been widely used on species of the genera Prunus and Vitis particularly rich in polyphenols and other enzyme-inhibiting compounds. The high quality of the sRNAs extracted from leaf or phloem tissues makes them suitable for all molecular biology reactions, in particular for next-generation sequencing library preparation. |
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Unknown | 3 | 30% |