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Mendeley readers
| Chapter title |
Double-Stranded RNA-Enriched Preparations to Identify Viroids by Next-Generation Sequencing
|
|---|---|
| Chapter number | 3 |
| Book title |
Viral Metagenomics
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7683-6_3 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7682-9, 978-1-4939-7683-6
|
| Authors |
Beatriz Navarro, Francesco Di Serio |
| Abstract |
Approaches based on next-generation sequencing (NGS) coupled with bioinformatics tools have been developed for detecting viruses and viroids infecting herbaceous and woody plants. Here we describe a protocol to extract nucleic acids from citrus bark and enrich them in double-stranded RNAs. These preparations can be efficiently used for generating cDNA libraries that, after pair-end sequencing and bioinformatics analyses, allow efficient identification of the viroids infecting the source plant. |
Mendeley readers
The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 7 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 2 | 29% |
| Student > Bachelor | 1 | 14% |
| Lecturer | 1 | 14% |
| Student > Master | 1 | 14% |
| Unknown | 2 | 29% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 3 | 43% |
| Biochemistry, Genetics and Molecular Biology | 2 | 29% |
| Unknown | 2 | 29% |