Chapter title |
Different Approaches to Discover Mycovirus Associated to Marine Organisms
|
---|---|
Chapter number | 8 |
Book title |
Viral Metagenomics
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7683-6_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7682-9, 978-1-4939-7683-6
|
Authors |
Luca Nerva, Giovanna C. Varese, Massimo Turina, Nerva, Luca, Varese, Giovanna C., Turina, Massimo |
Abstract |
Here we describe the protocols to characterize the virome associated to fungi isolated from marine organisms assessed on the seagrass Posidonia oceanica and on the marine animal Holothuria poli. We provide detailed protocols for fungal isolation, fungal growth, and total RNA extraction. Ribosomal RNA depletion, cDNA library synthesis and normalization, and sequencing runs on different platforms are part of the protocols that are generally outsourced and therefore are not described in this chapter. We describe, instead, how raw reads are assembled into contigs and how to search for putative viral sequences. Furthermore, we detail qualitative checks to infer the existence of the virus as a replicative biological entity. |
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Unknown | 1 | 100% |
Demographic breakdown
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 16 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 3 | 19% |
Student > Ph. D. Student | 3 | 19% |
Student > Master | 2 | 13% |
Lecturer | 1 | 6% |
Student > Doctoral Student | 1 | 6% |
Other | 4 | 25% |
Unknown | 2 | 13% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 7 | 44% |
Biochemistry, Genetics and Molecular Biology | 2 | 13% |
Environmental Science | 1 | 6% |
Unspecified | 1 | 6% |
Immunology and Microbiology | 1 | 6% |
Other | 0 | 0% |
Unknown | 4 | 25% |