Chapter title |
Methodology for Urine Peptidome Analysis Based on Nano-HPLC Coupled to Fourier Transform Ion Cyclotron Resonance Mass Spectrometry
|
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Chapter number | 20 |
Book title |
Peptidomics
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Published by |
Humana Press, New York, NY, February 2018
|
DOI | 10.1007/978-1-4939-7537-2_20 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7536-5, 978-1-4939-7537-2
|
Authors |
Alexey S. Kononikhin, Victoria A. Sergeeva, Anna E. Bugrova, Maria I. Indeykina, Natalia L. Starodubtseva, Vitaliy V. Chagovets, Igor A. Popov, Vladimir E. Frankevich, Patrick Pedrioli, Gennady T. Sukhikh, Eugene N. Nikolaev |
Abstract |
Urine is a sample of choice for noninvasive biomarkers search because it is easily available in large amounts and its molecular composition provides information on processes in the organism. The high potential of urine peptidomics has been demonstrated for clinical purpose. Several mass spectrometry based approaches have been successfully applied for urine peptidome analysis and potential biomarkers search. Summarizing literature data and our own experience we developed a protocol for comprehensive urine peptidome analysis. The technology includes several stages and consists of urine sample preparation by size exclusion chromatography and identification of featured peptides by nano-HPLC coupled to Fourier transform ion cyclotron resonance mass spectrometry, semiquantitative and statistical data analysis. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 11 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Other | 2 | 18% |
Lecturer > Senior Lecturer | 1 | 9% |
Professor | 1 | 9% |
Student > Master | 1 | 9% |
Professor > Associate Professor | 1 | 9% |
Other | 0 | 0% |
Unknown | 5 | 45% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 3 | 27% |
Agricultural and Biological Sciences | 2 | 18% |
Unknown | 6 | 55% |