Chapter title |
Quantitative Peptidomics with Isotopic and Isobaric Tags
|
---|---|
Chapter number | 9 |
Book title |
Peptidomics
|
Published by |
Humana Press, New York, NY, February 2018
|
DOI | 10.1007/978-1-4939-7537-2_9 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7536-5, 978-1-4939-7537-2
|
Authors |
Kurt Boonen, Wouter De Haes, Joris Van Houtven, Rik Verdonck, Geert Baggerman, Dirk Valkenborg, Liliane Schoofs |
Abstract |
In differential peptidomics, peptide profiles are compared between biological samples and the resulting expression levels are correlated to a phenotype of interest. This, in turn, allows us insight into how peptides may affect the phenotype of interest. In quantitative differential peptidomics, both label-based and label-free techniques are often employed. Label-based techniques have several advantages over label-free methods, primarily that labels allow for various samples to be pooled prior to liquid chromatography-mass spectrometry (LC-MS) analysis, reducing between-run variation. Here, we detail a method for performing quantitative peptidomics using stable amine-binding isotopic and isobaric tags. |
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