| Chapter title |
Identification of Endogenous Neuropeptides in the Nematode C. elegans Using Mass Spectrometry
|
|---|---|
| Chapter number | 18 |
| Book title |
Peptidomics
|
| Published by |
Springer New York, February 2018
|
| DOI | 10.1007/978-1-4939-7537-2_18 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7536-5, 978-1-4939-7537-2
|
| Authors |
Sven Van Bael, Samantha L. Edwards, Steven J. Husson, Liesbet Temmerman |
| Abstract |
The nematode Caenorhabditis elegans lends itself as an excellent model organism for peptidomics studies. Its ease of cultivation and quick generation time make it suitable for high-throughput studies. Adult hermaphrodites contain 959 somatic nuclei that are ordered in defined, differentiated tissues. The nervous system, with its 302 neurons, is probably the most known and studied endocrine tissue. Moreover, its neuropeptidergic signaling pathways display a large number of similarities with those observed in other metazoans. However, various other tissues have also been shown to express several neuropeptides. This includes the hypodermis, gonad, gut, and even muscle. Hence, whole mount peptidomics of C. elegans cultures provides an integral overview of peptidergic signaling between the different tissues of the entire organism. Here, we describe a peptidomics approach used for the identification of endogenous (neuro)peptides in C. elegans. Starting from a detailed peptide extraction procedure, we will outline the setup for an online liquid chromatography-mass spectrometry (LC-MS) analysis and describe subsequent data analysis approaches. |
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Mendeley readers
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| Professor | 2 | 5% |
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