Chapter title |
Vaccine Technologies for Veterinary Viral Diseases
|
---|---|
Chapter number | 7 |
Book title |
Vaccine Technologies for Veterinary Viral Diseases
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3008-1_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3007-4, 978-1-4939-3008-1
|
Authors |
Keil, Günther M, Pollin, Reiko, Müller, Claudia, Giesow, Katrin, Schirrmeier, Horst, Günther M. Keil, Reiko Pollin, Claudia Müller, Katrin Giesow, Horst Schirrmeier |
Abstract |
Recombinant baculo viruses based on Autographa californica multiple nuclear polyhedrosis virus carrying vertebrate cell active expression cassettes, so-called BacMam viruses, are increasingly used as gene delivery vectors for vaccination of animals against pathogens. Different approaches for generation of BacMams exist and a variety of transfer vectors to improve target protein expression in vivo have been constructed. Here we describe a use of transfer vector which contains an insect cell-restricted expression cassette for the green fluorescent protein and thus enables easy monitoring of BacMam virus rescue, fast plaque purification of recombinants and their convenient titer determination and which has been proven to be efficacious for gene delivery in vaccination/challenge experiments. |
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