Vaccine Technologies for Veterinary Viral Diseases
Humana Press, New York, NY
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Timeline
Mendeley readers
| Chapter title |
Generation and Selection of Orf Virus (ORFV) Recombinants
|
|---|---|
| Chapter number | 12 |
| Book title |
Vaccine Technologies for Veterinary Viral Diseases
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3008-1_12 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3007-4, 978-1-4939-3008-1
|
| Authors |
Hanns-Joachim Rziha, Jörg Rohde, Ralf Amann |
| Abstract |
Orf virus (ORFV) is an epitheliotropic poxvirus, which belongs to the genus Parapoxvirus. Among them the highly attenuated, apathogenic strain D1701-V is regarded as a promising candidate for novel virus vector vaccines. Our recent work demonstrated that those ORFV-based recombinants were able to induce protective, long-lasting immunity in various hosts that are non-permissive for ORFV. In this chapter we describe procedures for the generation, selection, propagation, and titration of ORFV recombinants as well as transgene detection by PCR or immunohistochemical staining. |
Mendeley readers
The data shown below were compiled from readership statistics for 15 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | 7% |
| Unknown | 14 | 93% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 4 | 27% |
| Student > Doctoral Student | 3 | 20% |
| Student > Bachelor | 2 | 13% |
| Professor | 1 | 7% |
| Unspecified | 1 | 7% |
| Other | 2 | 13% |
| Unknown | 2 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 4 | 27% |
| Veterinary Science and Veterinary Medicine | 2 | 13% |
| Immunology and Microbiology | 2 | 13% |
| Agricultural and Biological Sciences | 2 | 13% |
| Unspecified | 1 | 7% |
| Other | 2 | 13% |
| Unknown | 2 | 13% |