Chapter title |
Polycistronic Herpesvirus Amplicon Vectors for Veterinary Vaccine Development
|
---|---|
Chapter number | 13 |
Book title |
Vaccine Technologies for Veterinary Viral Diseases
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3008-1_13 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3007-4, 978-1-4939-3008-1
|
Authors |
Anita Felicitas Meier, Andrea Sara Laimbacher, Mathias Ackermann |
Abstract |
Heterologous virus-vectored vaccines, particularly those based on canarypox virus vectors, have established a firm place in preventive veterinary medicine. However, herpesvirus-based vaccines have paved the way for DIVA vaccines (discrimination of infected against vaccinated animals), which are particularly desirable for highly contagious livestock diseases that are otherwise combatted by culling of infected animals.In this chapter, we describe the design, the preparation, and the testing of a polycistronic herpesvirus amplicon vaccine against rotaviruses with a particular emphasis on generating heterologous virus-like particles for immunization. After the design, the procedure consists of three steps, first, transient expression of the construct in cell cultures, second, expression and antibody response in a mouse model, and third, application of the system to the desired host species. As a whole, the present information will facilitate the design of novel vaccines of veterinary interest from the designing process until pre-licensing. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 8 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 3 | 38% |
Student > Ph. D. Student | 2 | 25% |
Other | 1 | 13% |
Student > Doctoral Student | 1 | 13% |
Unknown | 1 | 13% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 3 | 38% |
Biochemistry, Genetics and Molecular Biology | 1 | 13% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 13% |
Nursing and Health Professions | 1 | 13% |
Medicine and Dentistry | 1 | 13% |
Other | 0 | 0% |
Unknown | 1 | 13% |