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Mendeley readers
Chapter title |
2D-DIGE Analysis of Eye Lens Proteins as a Measure of Cataract Formation
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Chapter number | 30 |
Book title |
Investigations of Early Nutrition Effects on Long-Term Health
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Published in |
Methods in molecular biology, January 2018
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DOI | 10.1007/978-1-4939-7614-0_30 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7613-3, 978-1-4939-7614-0
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Authors |
Paul C. Guest |
Abstract |
This chapter describes the basics of two-dimensional difference gel electrophoresis (2D-DIGE) for multiplex analysis of two distinct proteomes. The example given describes the analysis of male and female rat lens soluble proteins labeled with fluorescent Cy3 and Cy5 dyes in comparison to a pooled standard labeled with Cy2. After labeling the proteomes are mixed together and electrophoresed on the same 2D gels. Scanning the gels at wavelengths specific for each dye allows direct overlay the two different proteomes. Differences in abundance of specific protein spots can be determined through comparison to the pooled standard. |
Mendeley readers
The data shown below were compiled from readership statistics for 1 Mendeley reader of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Master | 1 | 100% |
Readers by discipline | Count | As % |
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Medicine and Dentistry | 1 | 100% |