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Mendeley readers
Chapter title |
Pulse-Chase Biosynthetic Radiolabeling of Pancreatic Islets to Measure Beta Cell Function
|
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Chapter number | 22 |
Book title |
Investigations of Early Nutrition Effects on Long-Term Health
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Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7614-0_22 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7613-3, 978-1-4939-7614-0
|
Authors |
Paul C. Guest |
Abstract |
Pulse-chase radiolabeling of cells with radioactive amino acids is a common method for studying the biosynthesis of proteins. The labeled proteins can then be immunoprecipitated and analyzed by electrophoresis and gel imaging techniques. This chapter presents a protocol for the biosynthetic labeling and immunoprecipitation of pancreatic islet proteins which are known to be affected in disorders such as diabetes, obesity, and metabolic syndrome. |
Mendeley readers
The data shown below were compiled from readership statistics for 1 Mendeley reader of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Lecturer > Senior Lecturer | 1 | 100% |
Readers by discipline | Count | As % |
---|---|---|
Chemical Engineering | 1 | 100% |