Chapter title |
In Vivo Electrical Stimulation for the Assessment of Skeletal Muscle Contractile Function in Murine Models
|
---|---|
Chapter number | 26 |
Book title |
Investigations of Early Nutrition Effects on Long-Term Health
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7614-0_26 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7613-3, 978-1-4939-7614-0
|
Authors |
Kaio F. Vitzel, Marco A. Fortes, Gabriel Nasri Marzuca-Nassr, Maria V. M. Scervino, Carlos H. Pinheiro, Leonardo R. Silveira, Rui Curi |
Abstract |
Skeletal muscle electrical stimulation is commonly used for clinical purposes, assisting recovery, preservation, or even improvement of muscle mass and function in healthy and pathological conditions. Additionally, it is a useful research tool for evaluation of skeletal muscle contractile function. It may be applied in vitro, using cell culture or isolated fibers/muscles, and in vivo, using human subjects or animal models (neuromuscular electrical stimulation - NMES). This chapter focuses on the electrical stimulation of the sciatic nerve as a research method for evaluation of the contractile properties of murine hind limb muscles. Variations of this protocol allow for the assessment of muscle force, fatigue resistance, contraction and relaxation times, and can be used as a model of contraction-induced muscle injury, reactive oxygen species production, and muscle adaptation to contractile activity. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 39 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 8 | 21% |
Student > Master | 5 | 13% |
Student > Ph. D. Student | 4 | 10% |
Researcher | 3 | 8% |
Student > Doctoral Student | 2 | 5% |
Other | 6 | 15% |
Unknown | 11 | 28% |
Readers by discipline | Count | As % |
---|---|---|
Sports and Recreations | 8 | 21% |
Nursing and Health Professions | 5 | 13% |
Medicine and Dentistry | 5 | 13% |
Biochemistry, Genetics and Molecular Biology | 3 | 8% |
Agricultural and Biological Sciences | 2 | 5% |
Other | 5 | 13% |
Unknown | 11 | 28% |