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Mendeley readers
Chapter title |
Time-Resolved Fluorescence Assays for Quantification of Insulin Precursors in Plasma and Serum
|
---|---|
Chapter number | 33 |
Book title |
Investigations of Early Nutrition Effects on Long-Term Health
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Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7614-0_33 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7613-3, 978-1-4939-7614-0
|
Authors |
Kevin Taylor, Ian Halsall, Paul C. Guest, Keith Burling |
Abstract |
In metabolic diseases such as obesity and type 2 diabetes mellitus, the conversion of proinsulin to mature insulin can be impaired. This could mean that insulin molecules with lower activity toward the insulin receptor can be released under conditions of high metabolic demand, resulting in an inadequate glucoregulatory response. The chapter describes a fluorescent monoclonal antibody-based protocol for measurement of human proinsulin and the proinsulin conversion intermediates (split proinsulins). An example assay is presented using serum from non-diabetic, normal body mass index individuals. |
Mendeley readers
The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 4 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 2 | 50% |
Student > Bachelor | 1 | 25% |
Unknown | 1 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 2 | 50% |
Unknown | 2 | 50% |