| Chapter title |
The Aortic Ring Assay and Its Use for the Study of Tumor Angiogenesis
|
|---|---|
| Chapter number | 6 |
| Book title |
Tumor Angiogenesis Assays
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3999-2_6 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3997-8, 978-1-4939-3999-2
|
| Authors |
Alfred C. Aplin, Roberto F. Nicosia |
| Abstract |
This chapter describes protocols developed in our laboratory to prepare and analyze angiogenic cultures of rat aorta. Rings of rat aorta cultured in collagen gels produce neovessel outgrowths which reproduce ex vivo key steps of the angiogenic process including endothelial migration, proliferation, proteolytic digestion of the extracellular matrix, capillary tube formation, pericyte recruitment, and vascular regression. The angiogenic response of aortic explants can be stimulated with growth factors or inhibited with anti-angiogenic molecules. Aortic ring cultures can also be used to study tumor angiogenesis. Protocols outlined in this chapter describe how this assay can be modified to investigate the angiogenic activity of cancer cells. |
Mendeley readers
Geographical breakdown
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|---|---|---|
| Unknown | 9 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 3 | 33% |
| Student > Master | 2 | 22% |
| Student > Bachelor | 1 | 11% |
| Other | 1 | 11% |
| Student > Doctoral Student | 1 | 11% |
| Other | 1 | 11% |
| Readers by discipline | Count | As % |
|---|---|---|
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| Engineering | 2 | 22% |
| Biochemistry, Genetics and Molecular Biology | 1 | 11% |