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Laser Capture Microdissection

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Cover of 'Laser Capture Microdissection'

Table of Contents

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    Book Overview
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    Chapter 1 Laser Capture Microdissection: Insights into Methods and Applications
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    Chapter 2 Laser Microdissection-Based Microproteomics of Formalin-Fixed and Paraffin-Embedded (FFPE) Tissues
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    Chapter 3 Laser Microdissection Workflow for Isolating Nucleic Acids from Fixed and Frozen Tissue Samples
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    Chapter 4 Protocol for the Analysis of Laser Capture Microdissected Fresh-Frozen Tissue Homogenates by Silver-Stained 1D SDS-PAGE
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    Chapter 5 Next-Generation Sequencing Analysis of Laser-Microdissected Formalin-Fixed and Paraffin-Embedded (FFPE) Tissue Specimens
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    Chapter 6 Adaptation of Laser Microdissection Technique to Nanostring RNA Analysis in the Study of a Spontaneous Metastatic Mammary Carcinoma Mouse Model
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    Chapter 7 Laser Capture Microdissection as a Tool to Study the Mucosal Immune Response in Celiac Disease
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    Chapter 8 Laser Capture Microdissection and Isolation of High-Quality RNA from Frozen Endometrial Tissue
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    Chapter 9 Laser Microdissection for Human Papillomavirus (HPV) Genotyping Attribution and Methylation Pattern Analyses of Squamous Intraepithelial Lesions
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    Chapter 10 Laser Capture Microdissection and Transcriptional Analysis of Sub-Populations of the Osteoblast Lineage from Undecalcified Bone
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    Chapter 11 Cell Type-Specific Laser Capture Microdissection for Gene Expression Profiling in the Human Brain
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    Chapter 12 The Isolation of Pure Populations of Neurons by Laser Capture Microdissection: Methods and Application in Neuroscience
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    Chapter 13 Laser Capture Microdissection in Traumatic Brain Injury Research: Obtaining Hippocampal Subregions and Pools of Injured Neurons for Genomic Analyses
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    Chapter 14 Isolation of Distinct Types of Neurons from Fresh Brain Tissue Using Laser Microdissection in Combination with High-Performance Liquid Chromatography—Mass Spectrometry
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    Chapter 15 Immuno-Guided Laser-Capture Microdissection of Glial Cells for mRNA Analysis
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    Chapter 16 Immuno-Laser-Capture Microdissection for the Isolation of Enriched Glial Populations from Frozen Post-Mortem Human Brain
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    Chapter 17 Laser-Capture Microdissection for the Analysis of Rat and Human Spinal Cord Ependyma by qPCR
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    Chapter 18 Isolation of Amyloid Plaques and Neurofibrillary Tangles from Archived Alzheimer’s Disease Tissue Using Laser-Capture Microdissection for Downstream Proteomics
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    Chapter 19 Cell-Specific RNA Quantification in Human SN DA Neurons from Heterogeneous Post-mortem Midbrain Samples by UV-Laser Microdissection and RT-qPCR
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    Chapter 20 Laser-Capture Microdissection for Layer-Specific Analysis of Enteric Ganglia
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    Chapter 21 A Laser Microdissection–Liquid Chromatography–Tandem Mass Spectrometry Workflow for Post-mortem Analysis of Brain Tissue
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    Chapter 22 Laser-Capture Microdissection and RNA Extraction from Perfusion-Fixed Cartilage and Bone Tissue from Mice Implanted with Human iPSC-Derived MSCs in a Calvarial Defect Model
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    Chapter 23 Laser Capture Microdissection-Based RNA-Seq of Barley Grain Tissues
Attention for Chapter 6: Adaptation of Laser Microdissection Technique to Nanostring RNA Analysis in the Study of a Spontaneous Metastatic Mammary Carcinoma Mouse Model
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Chapter title
Adaptation of Laser Microdissection Technique to Nanostring RNA Analysis in the Study of a Spontaneous Metastatic Mammary Carcinoma Mouse Model
Chapter number 6
Book title
Laser Capture Microdissection
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7558-7_6
Pubmed ID
Book ISBNs
978-1-4939-7557-0, 978-1-4939-7558-7
Authors

Nadia P. Castro, Yelena G. Golubeva, Castro, Nadia P., Golubeva, Yelena G.

Abstract

The mouse model characterized by spontaneous lung metastasis from JygMC (A) cells closely resembles the human triple negative breast cancer (TNBC) subtype. The primary tumors morphologically present both epithelial and spindle-like cells, but metastases in lung parenchyma display only adenocarcinoma properties. In the study of molecular signatures, laser capture microdissection (LCM) on frozen tissue sections was used to separate the following regions of interest: the epithelial-mesenchymal transition (EMT), mesenchymal-epithelial transition (MET), carcinoma, lung metastases, normal mammary gland and normal lung parenchyma. NanoString was selected for the study of molecular signatures in LCM targets as a reliable downstream gene expression platform allowing analysis of tissue lysates without RNA extraction and amplification. This chapter provides detailed protocols for the collection of tissue, LCM sample preparation and dissection, production of lysates, extraction, and quality control of RNA for NanoString analysis, as well as the methodology of Nanostring gene expression profiling experiment.

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 9 100%

Demographic breakdown

Readers by professional status Count As %
Other 2 22%
Librarian 1 11%
Researcher 1 11%
Student > Ph. D. Student 1 11%
Unknown 4 44%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 22%
Immunology and Microbiology 1 11%
Medicine and Dentistry 1 11%
Engineering 1 11%
Unknown 4 44%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 January 2018.
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#20,459,801
of 23,016,919 outputs
Outputs from Methods in molecular biology
#9,945
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Outputs of similar age
#378,196
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Outputs of similar age from Methods in molecular biology
#1,193
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