Chapter title |
Stable Expression of Epigenome Editors via Viral Delivery and Genomic Integration
|
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Chapter number | 11 |
Book title |
Epigenome Editing
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7774-1_11 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7773-4, 978-1-4939-7774-1
|
Authors |
Carolin Kroll, Philipp Rathert |
Abstract |
The advent of precise genomic targeting systems has revolutionized epigenome editing through fusion of epigenetic effector proteins with engineered DNA-binding proteins. However, the delivery of plasmid DNA to express these fusion proteins via conventional transient transfection has certain consequences which need to be considered during the experimental design. Transient transfection achieves peak gene expression between 24 and 96 h post-transfection after which the foreign gene is lost through cell division and degradation. The use of cell lines stably expressing the effector fusion protein of interest provides several advantages compared to standard transfection methods, and the most suitable means for creating these cell lines was found to be viral delivery followed by stable integration of the transgenes into the host genome. Here we describe a practical protocol to generate murine cell lines stably expressing fusion proteins of chromatin regulators and DNA-binding proteins using a retroviral murine stem cell virus (MSCV)-based vector system. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 6 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 1 | 17% |
Student > Bachelor | 1 | 17% |
Researcher | 1 | 17% |
Unknown | 3 | 50% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 3 | 50% |
Unknown | 3 | 50% |