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Chapter title |
Cell-to-Cell Transcription Variability as Measured by Single-Molecule RNA FISH to Detect Epigenetic State Switching
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Chapter number | 21 |
Book title |
Epigenome Editing
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Published in |
Methods in molecular biology, January 2018
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DOI | 10.1007/978-1-4939-7774-1_21 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7773-4, 978-1-4939-7774-1
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Authors |
William Beckman, Ilona M. Vuist, Hermannus Kempe, Pernette J. Verschure |
Abstract |
Single-molecule RNA fluorescent in situ hybridization (smRNA FISH) allows for the visualization, localization, and quantification of RNA transcripts within individual cells and tissues using custom-designed fluorescently labeled oligonucleotide probes. Here we describe a protocol for the preparation, imaging, and analysis of a smRNA FISH experiment that can be applied to any RNA of choice. We also provide insights as to how this powerful tool can be used to study epigenetic regulation, for example, following the epigenetic editing of genes. |
Mendeley readers
The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
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Unknown | 4 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 4 | 100% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 3 | 75% |
Agricultural and Biological Sciences | 1 | 25% |