Chapter title |
A Method for High-Throughput Analysis of Chronological Aging in Schizosaccharomyces pombe.
|
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Chapter number | 7 |
Book title |
Chemical Biology
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Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2269-7_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2268-0, 978-1-4939-2269-7
|
Authors |
Jessica Stephan, Ann E Ehrenhofer-Murray, Ann E. Ehrenhofer-Murray, Stephan, Jessica, Ehrenhofer-Murray, Ann E. |
Abstract |
The measurement of chronological life span (CLS) in Schizosaccharomyces pombe is traditionally performed by plating back aliquots of aging liquid cultures on solid medium and counting the number of colony-forming units (CFU). However, this method is labor and cost intensive and therefore not amenable to high-throughput screening. Here, we describe a simple method for CLS measurement using aging minicultures in microtiter plates and batch plate-back for the determination of culture viability. This assay can be used to screen a large number of strains, conditions, or compounds in parallel for effects on aging. |
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Mendeley readers
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