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Chapter title |
A Mix-and-Measure Assay for Determining the Activation Status of Endogenous Cdc42 in Cytokine-stimulated Macrophage Cell Lysates.
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Chapter number | 15 |
Book title |
Cytokine Bioassays
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Published in |
Methods in molecular biology, June 2014
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DOI | 10.1007/978-1-4939-0928-5_15 |
Pubmed ID | |
Book ISBNs |
978-1-4939-0927-8, 978-1-4939-0928-5
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Authors |
Miskolci V, Spiering D, Cox D, Hodgson L, Veronika Miskolci, Désirée Spiering, Dianne Cox, Louis Hodgson |
Abstract |
Cytokine stimulations of leukocytes many times result in transient activation of the p21 Rho family of small GTPases. The role of these molecules during cell migration and chemotaxis is well established. The traditional approach to study the activation dynamics of these proteins involves affinity pull-downs that are often cumbersome and prone to errors. Here, we describe a reagent and a method of simple "mix-and-measure" approach useful for determining the activation status of endogenous Cdc42 GTPase from cell lysates. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 January 2015.
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#20,249,662
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#9,869
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Outputs of similar age
#193,560
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Outputs of similar age from Methods in molecular biology
#53
of 110 outputs
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