| Chapter title |
Inflammatory Caspases: Activation and Cleavage of Gasdermin-D In Vitro and During Pyroptosis
|
|---|---|
| Chapter number | 9 |
| Book title |
Innate Immune Activation
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7519-8_9 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7518-1, 978-1-4939-7519-8
|
| Authors |
Yue Zhao, Jianjin Shi, Feng Shao |
| Abstract |
Gasdermin-D (also known as GSDMD), the newly identified executioner of pyroptotic cell death, is cleaved by activated caspase-1 downstream of canonical inflammasome activation or caspase-4, 5, and 11 upon their ligation and activation by cytosolic LPS. Upon a single cleavage between the two domains in Gasdermin-D, the N-terminal domain binds to membrane lipids and lyses cells by forming pores of an inner diameter of 10-14 nm within the membrane. The inter-domain cleavage of Gasdermin-D is a reliable marker for the activation of inflammatory caspases and cell pyroptosis. Here, we describe the methods for examining Gasdermin-D cleavage by activated inflammatory caspases in vitro and upon inflammasome activation in vivo. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 52 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 11 | 21% |
| Student > Master | 9 | 17% |
| Researcher | 8 | 15% |
| Student > Bachelor | 2 | 4% |
| Student > Postgraduate | 2 | 4% |
| Other | 4 | 8% |
| Unknown | 16 | 31% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 13 | 25% |
| Immunology and Microbiology | 9 | 17% |
| Medicine and Dentistry | 6 | 12% |
| Agricultural and Biological Sciences | 5 | 10% |
| Veterinary Science and Veterinary Medicine | 1 | 2% |
| Other | 2 | 4% |
| Unknown | 16 | 31% |