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Innate Immune Activation

Overview of attention for book
Cover of 'Innate Immune Activation'

Table of Contents

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    Book Overview
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    Chapter 1 Emerging Concepts in Innate Immunity
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    Chapter 2 Bioinformatic Assessment of Macrophage Activation by the Innate Immune System
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    Chapter 3 Generation of Genetic Knockouts in Myeloid Cell Lines Using a Lentiviral CRISPR/Cas9 System
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    Chapter 4 Modeling Primary Human Monocytes with the Trans–Differentiation Cell Line BLaER1
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    Chapter 5 Measurement of NF-κB Activation in TLR-Activated Macrophages
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    Chapter 6 Biochemical Isolation of the Myddosome from Murine Macrophages
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    Chapter 7 Generation of Innate Immune Reporter Cells Using Retroviral Transduction
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    Chapter 8 Examining Myddosome Formation by Luminescence-Based Mammalian Interactome Mapping (LUMIER)
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    Chapter 9 Inflammatory Caspases: Activation and Cleavage of Gasdermin-D In Vitro and During Pyroptosis
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    Chapter 10 Detection of ASC Speck Formation by Flow Cytometry and Chemical Cross-linking
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    Chapter 11 Measuring Innate Immune Responses to Bacterial Viability
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    Chapter 12 Methods to Study Cell Swelling-Induced Inflammasome Activation
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    Chapter 13 Detecting Release of Bacterial dsDNA into the Host Cytosol Using Fluorescence Microscopy
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    Chapter 14 Quantitative Proteomics of Secreted Proteins
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    Chapter 15 Simultaneous Detection of Cellular Viability and Interleukin-1β Secretion from Single Cells by ELISpot
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    Chapter 16 Detection and Quantification of MAVS Aggregation via Confocal Microscopy
Attention for Chapter 7: Generation of Innate Immune Reporter Cells Using Retroviral Transduction
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Chapter title
Generation of Innate Immune Reporter Cells Using Retroviral Transduction
Chapter number 7
Book title
Innate Immune Activation
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7519-8_7
Pubmed ID
Book ISBNs
978-1-4939-7518-1, 978-1-4939-7519-8
Authors

Yamel Cardona Gloria, Eicke Latz, Dominic De Nardo, Cardona Gloria, Yamel, Latz, Eicke, De Nardo, Dominic

Abstract

Innate immune cells are notoriously difficult to transfect; however, retroviruses can be used to stably integrate genes of interest into the host genome of primary or immortalized immune cells resulting in the generation of reporter cells. Here, we provide a detailed protocol covering the production of retroviruses, retroviral infection of innate immune target cells (including isolation and differentiation of murine bone marrow cells to macrophages), and several methods for enrichment of positively transduced cells.

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 16 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 16 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 4 25%
Professor 2 13%
Student > Ph. D. Student 2 13%
Student > Master 2 13%
Student > Bachelor 1 6%
Other 0 0%
Unknown 5 31%
Readers by discipline Count As %
Agricultural and Biological Sciences 5 31%
Biochemistry, Genetics and Molecular Biology 3 19%
Immunology and Microbiology 2 13%
Unknown 6 38%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 July 2018.
All research outputs
#20,453,782
of 23,009,818 outputs
Outputs from Methods in molecular biology
#9,941
of 13,157 outputs
Outputs of similar age
#378,135
of 442,295 outputs
Outputs of similar age from Methods in molecular biology
#1,193
of 1,498 outputs
Altmetric has tracked 23,009,818 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,157 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 442,295 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,498 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.