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Kidney Research

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Cover of 'Kidney Research'

Table of Contents

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    Book Overview
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    Chapter 1 Isolation and Primary Culture of Murine Podocytes with Proven Origin
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    Chapter 2 Kidney Research
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    Chapter 3 Isolation and Propagation of Rat Peritoneal Mesothelial Cells
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    Chapter 4 Rat Models of Acute and/or Chronic Peritoneal Injuries Including Peritoneal Fibrosis and Peritoneal Dialysis Complications
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    Chapter 5 Renal Sympathetic Denervation in Rats
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    Chapter 6 Decellularization of Rat Kidneys to Produce Extracellular Matrix Scaffolds
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    Chapter 7 Use of Cationized Ferritin Nanoparticles to Measure Renal Glomerular Microstructure with MRI
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    Chapter 8 Biopsychronology: A Method Using Live Tissue Staining to Image Cell Function in the Kidney
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    Chapter 9 Prolonged and Continuous Measurement of Kidney Oxygenation in Conscious Rats.
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    Chapter 10 Magnetic Resonance Imaging (MRI) Analysis of Ischemia/Reperfusion in Experimental Acute Renal Injury.
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    Chapter 11 Assessment of Renal Hemodynamics and Oxygenation by Simultaneous Magnetic Resonance Imaging (MRI) and Quantitative Invasive Physiological Measurements.
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    Chapter 12 Intravital Multiphoton Imaging of the Kidney: Tubular Structure and Metabolism
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    Chapter 13 Vascular Calcification in Uremia: New-Age Concepts about an Old-Age Problem
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    Chapter 14 An In Vitro Murine Model of Vascular Smooth Muscle Cell Mineralization
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    Chapter 15 The Isolation and Quantitation of Fetuin-A-Containing Calciprotein Particles from Biological Fluids.
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    Chapter 16 Combining Near Infrared Fluorescent Imaging for Calcification and Inflammation in Vascular Tissue Samples Ex Vivo
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    Chapter 17 Kidney Research
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    Chapter 18 Kidney Research
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    Chapter 19 Kidney Research
Attention for Chapter 3: Isolation and Propagation of Rat Peritoneal Mesothelial Cells
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Chapter title
Isolation and Propagation of Rat Peritoneal Mesothelial Cells
Chapter number 3
Book title
Kidney Research
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3353-2_3
Pubmed ID
Book ISBNs
978-1-4939-3351-8, 978-1-4939-3353-2
Authors

Qin Zhou, Xueqing Yu

Abstract

With the development of peritoneal dialysis in many countries, there has been much interest in the cell biology of peritoneal mesothelial cells. In this chapter we describe a reliable and reproducible method for the culture of rat primary mesothelial cells (RPMCs). This chapter outlines how to isolate mesothelial cells from rat peritoneum. The subculture of primary peritoneal mesothelial cells and the characterization by immunofluorescence is also described in detail.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 3 100%

Demographic breakdown

Readers by professional status Count As %
Other 1 33%
Student > Ph. D. Student 1 33%
Student > Bachelor 1 33%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 1 33%
Nursing and Health Professions 1 33%
Medicine and Dentistry 1 33%