Chapter title |
Isolation and Primary Culture of Murine Podocytes with Proven Origin
|
---|---|
Chapter number | 1 |
Book title |
Kidney Research
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3353-2_1 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3351-8, 978-1-4939-3353-2
|
Authors |
Bart Smeets, Nazanin Kabgani, Marcus J. Moeller, Smeets, Bart, Kabgani, Nazanin, Moeller, Marcus J. |
Abstract |
Genetic studies on hereditary kidney diseases and in vivo experimental model studies have revealed a critical role for the podocyte in glomerular function and disease. Primary podocyte cultures as well as immortalized podocyte cell lines have been used extensively to study podocyte function. Although, primary cells often more closely resemble the in vivo cells, they may have only a finite replicative life span before they reach senescence. Therefore, the success of studies using primary cell cultures depends on standardized isolation and culture protocols that allow reproducible generation of stable primary cultures.This chapter describes the isolation of primary podocytes with a proven origin using the novel technology of cell-specific genetic tagging. Podocytes are isolated from glomeruli from a podocyte-specific transgenic reporter mouse. The podocyte-specific reporter gene beta-galactosidase is used to identify and specifically isolate the labeled podocytes from other glomerular cells by FACS. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 7 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 3 | 43% |
Researcher | 1 | 14% |
Other | 1 | 14% |
Student > Doctoral Student | 1 | 14% |
Unknown | 1 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 4 | 57% |
Biochemistry, Genetics and Molecular Biology | 2 | 29% |
Unknown | 1 | 14% |