Chapter title |
Use of Cationized Ferritin Nanoparticles to Measure Renal Glomerular Microstructure with MRI
|
---|---|
Chapter number | 7 |
Book title |
Kidney Research
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3353-2_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3351-8, 978-1-4939-3353-2
|
Authors |
Kevin M. Bennett, Scott C. Beeman, Edwin J. Baldelomar, Min Zhang, Teresa Wu, Bradley D. Hann, John F. Bertram, Jennifer R. Charlton |
Abstract |
Magnetic resonance imaging (MRI) is becoming important for whole-kidney assessment of glomerular morphology, both in vivo and ex vivo. MRI-based renal morphological measurements can be made in intact organs and allow direct measurements of every perfused glomerulus. Cationic ferritin (CF) is used as a superparamagnetic contrast agent for MRI. CF binds to the glomerular basement membrane after intravenous injection, allowing direct, whole-kidney measurements of glomerular number, volume, and volume distribution. Here we describe the production, testing, and use of CF as an MRI contrast agent for quantitative glomerular morphology in intact mouse, rat, and human kidneys. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 9 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 3 | 33% |
Professor > Associate Professor | 2 | 22% |
Lecturer | 1 | 11% |
Student > Doctoral Student | 1 | 11% |
Other | 1 | 11% |
Other | 0 | 0% |
Unknown | 1 | 11% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 2 | 22% |
Medicine and Dentistry | 2 | 22% |
Physics and Astronomy | 1 | 11% |
Engineering | 1 | 11% |
Unknown | 3 | 33% |