Chapter title |
Genomic Approaches to Analyze Alternative Splicing, A Key Regulator of Transcriptome and Proteome Diversity in Brachypodium distachyon
|
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Chapter number | 7 |
Book title |
Brachypodium Genomics
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7278-4_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7276-0, 978-1-4939-7278-4
|
Authors |
Sonia Irigoyen, Renesh H. Bedre, Karen-Beth G. Scholthof, Kranthi K. Mandadi, Irigoyen, Sonia, Bedre, Renesh H., Scholthof, Karen-Beth G., Mandadi, Kranthi K. |
Abstract |
Alternative splicing (AS) promotes transcriptome and proteome diversity in plants, which influences growth and development, and host responses to stress. Advancements in next-generation sequencing, bioinformatics, and computational biology tools have allowed biologists to investigate AS landscapes on a genome-wide scale in several plant species. Furthermore, the development of Brachypodium distachyon (Brachypodium) as a model system for grasses has facilitated comparative studies of AS within the Poaceae. These analyses revealed a plethora of genes in several biological processes that are alternatively spliced and identified conserved AS patterns among monocot and dicot plants. In this chapter, using a Brachypodium-virus pathosystem as a research template, we provide an overview of genomic and bioinformatic tools that can be used to investigate constitutive and alternative splicing in plants. |
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