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Brachypodium Genomics

Overview of attention for book
Brachypodium Genomics
Humana Press, New York, NY

Table of Contents

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    Book Overview
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    Chapter 1 Methods for Cytogenetic Chromosome Barcoding and Chromosome Painting in Brachypodium distachyon and Its Relative Species
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    Chapter 2 Transcriptional and Posttranscriptional Regulation of Drought Stress Treatments in Brachypodium Leaves
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    Chapter 3 Brachypodium distachyon Long Noncoding RNAs: Genome-Wide Identification and Expression Analysis
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    Chapter 4 A Highly Efficient and Reproducible Fusarium spp. Inoculation Method for Brachypodium distachyon
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    Chapter 5 Tissue Culture (Somatic Embryogenesis)-Induced Tnt1 Retrotransposon-Based Mutagenesis in Brachypodium distachyon
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    Chapter 6 Methods for Xyloglucan Structure Analysis in Brachypodium distachyon
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    Chapter 7 Genomic Approaches to Analyze Alternative Splicing, A Key Regulator of Transcriptome and Proteome Diversity in Brachypodium distachyon
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    Chapter 8 Information Resources for Functional Genomics Studies in Brachypodium distachyon
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    Chapter 9 Methods for Functional Transgenics: Development of Highly Efficient Transformation Protocol in Brachypodium and Its Suitability for Advancing Brachypodium Transgenics
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    Chapter 10 Molecular Markers in Whole Genome Evolution of Brachypodium
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    Chapter 11 Estimate Codon Usage Bias Using Codon Usage Analyzer (CUA)
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    Chapter 12 Identification of Pseudogenes in Brachypodium distachyon Chromosomes
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    Chapter 13 TILLING in Brachypodium distachyon
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    Chapter 14 Method for the Large-Scale Identification of phasiRNAs in Brachypodium distachyon
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    Chapter 15 Evaluation of Genome-Wide Markers and Orthologous Markers in Brachypodium distachyon
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    Chapter 16 Protocol for Coexpression Network Construction and Stress-Responsive Expression Analysis in Brachypodium
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    Chapter 17 Whole Genome DNA Methylation Analysis Using Next-Generation Sequencing (BS-seq)
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    Chapter 18 Application of Tissue Culture and Transformation Techniques in Model Species Brachypodium distachyon
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    Chapter 19 Erratum to: Whole Genome DNA Methylation Analysis of Brachypodium distachyon Using Next-Generation Sequencing (BS-seq)
Attention for Chapter 2: Transcriptional and Posttranscriptional Regulation of Drought Stress Treatments in Brachypodium Leaves
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Chapter title
Transcriptional and Posttranscriptional Regulation of Drought Stress Treatments in Brachypodium Leaves
Chapter number 2
Book title
Brachypodium Genomics
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7278-4_2
Pubmed ID
Book ISBNs
978-1-4939-7276-0, 978-1-4939-7278-4
Authors

Edoardo Bertolini, Mario Enrico Pè, Erica Mica

Abstract

Plant sensing drought stress conditions activate complex molecular networks leading to a rapid reprogramming of plant physiology and metabolism, in order to survive in suboptimal conditions.Here, we describe a standardized in vivo soil drought assay to investigate the effects of drought stress on leaf growth. Since it is now clear that stress responses can be specific to developmental stages and cell types, we describe a procedure to dissect the leaf in three distinct areas in order to study transcriptional and posttranscriptional gene regulation on both organ and cellular levels. Noncoding RNAs, both small RNAs and long noncoding RNAs, are emerging to be deeply involved in abiotic stress responses, acting as molecular switches, interconnecting different response pathways. Here, we illustrate the methodology that has been used to identify miRNAs involved in drought response and to analyze the modulation of expression of their putative targets, in order to gain a complete picture of transcriptional and posttranscriptional regulation driven by noncoding RNAs.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 4 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 1 25%
Researcher 1 25%
Other 1 25%
Unknown 1 25%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 1 25%
Agricultural and Biological Sciences 1 25%
Unknown 2 50%