Chapter title |
Evaluation of Genome-Wide Markers and Orthologous Markers in Brachypodium distachyon
|
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Chapter number | 15 |
Book title |
Brachypodium Genomics
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7278-4_15 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7276-0, 978-1-4939-7278-4
|
Authors |
Gaurav Sablok, Suresh B. Mudunuri, Korneliya Gudys, Kranthi Chennamsetti, G. P. Saradhi Varma, Miroslaw Kwasniewski |
Abstract |
Molecular markers play an important role in identifying the species variation, characterizing the genic diversity, and also linking the identified markers to trait of interest. Genome- and transcriptome-derived molecular markers have been widely used to understand the geographical diversity and have also played a major role in the development of high-density linkage maps. In the present protocol, we present a detailed protocol on bioinformatics approaches towards the whole-genome and transcriptome-assisted simple sequence repeats (SSRs) marker mining in Brachypodium distachyon and identification of orthologus SSRs and their validation in Brachypodium ecotypes. We also present a protocol for the validation of the identified markers. |
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