| Chapter title |
Tissue Culture (Somatic Embryogenesis)-Induced Tnt1 Retrotransposon-Based Mutagenesis in Brachypodium distachyon
|
|---|---|
| Chapter number | 5 |
| Book title |
Brachypodium Genomics
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7278-4_5 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7276-0, 978-1-4939-7278-4
|
| Authors |
Upinder S. Gill, Juan C. Serrani-Yarce, Hee-Kyung Lee, Kirankumar S. Mysore |
| Abstract |
Brachypodium distachyon is a model grass species for economically important cereal crops. Efforts are in progress to develop useful functional genomic resources in Brachypodium. A tobacco retrotransposon, Tnt1, has been used successfully in recent past to generate insertional mutagenesis in several dicot plant species. Tnt1 retrotransposon replicates, transposes, and inserts at multiple random genomic locations in the plant genome. Transposition occurs only during somatic embryogenesis but not during seed transmission. We developed Brachypodium transgenic plants that can express the Tnt1 element. Here, we describe an efficient tissue culture-based approach to generate Tnt1 insertional mutant population using transgenic Brachypodium line expressing the Tnt1 retrotransposon. |
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|---|---|---|
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Demographic breakdown
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Mendeley readers
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|---|---|---|
| Unknown | 7 | 100% |
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| Unspecified | 1 | 14% |
| Student > Ph. D. Student | 1 | 14% |
| Researcher | 1 | 14% |
| Lecturer | 1 | 14% |
| Student > Master | 1 | 14% |
| Other | 0 | 0% |
| Unknown | 2 | 29% |
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| Agricultural and Biological Sciences | 2 | 29% |
| Unspecified | 1 | 14% |
| Computer Science | 1 | 14% |
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| Unknown | 2 | 29% |