Chapter title |
A Highly Efficient and Reproducible Fusarium spp. Inoculation Method for Brachypodium distachyon
|
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Chapter number | 4 |
Book title |
Brachypodium Genomics
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7278-4_4 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7276-0, 978-1-4939-7278-4
|
Authors |
Anuj Rana, Aneesh Karunakaran, Timothy L. Fitzgerald, Rosalie Sabburg, Elizabeth A. B. Aitken, Robert J. Henry, Jonathan J. Powell, Kemal Kazan |
Abstract |
Fusarium spp. are devastating fungal pathogens which cause significant losses in many cereal crops like wheat, maize, and barley. Genetic improvement of disease resistance requires an improved understanding of defense-associated processes operating in the host in response to an attack by Fusarium spp. Brachypodium distachyon is emerging as a model where host-cereal-infecting pathogen interactions can be studied conveniently. However, this requires developing an efficient infection assay that facilitates quick screening of germplasm (e.g., mutant lines). Here, we provide an efficient and reproducible Fusarium infection assay for Brachypodium. We believe this method will help further develop Brachypodium as a model for genetic improvement of disease resistance in cereals against Fusarium pathogens. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 4 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 1 | 25% |
Student > Doctoral Student | 1 | 25% |
Unknown | 2 | 50% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 1 | 25% |
Agricultural and Biological Sciences | 1 | 25% |
Unknown | 2 | 50% |