| Chapter title |
Detection of DNA Methylation by Whole-Genome Bisulfite Sequencing
|
|---|---|
| Chapter number | 11 |
| Book title |
Maize
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7315-6_11 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7314-9, 978-1-4939-7315-6
|
| Authors |
Qing Li, Peter J. Hermanson, Nathan M. Springer |
| Abstract |
DNA methylation plays an important role in the regulation of the expression of transposons and genes. Various methods have been developed to assay DNA methylation levels. Bisulfite sequencing is considered to be the "gold standard" for single-base resolution measurement of DNA methylation levels. Coupled with next-generation sequencing, whole-genome bisulfite sequencing (WGBS) allows DNA methylation to be evaluated at a genome-wide scale. Here, we described a protocol for WGBS in plant species with large genomes. This protocol has been successfully applied to assay genome-wide DNA methylation levels in maize and barley. This protocol has also been successfully coupled with sequence capture technology to assay DNA methylation levels in a targeted set of genomic regions. |
Mendeley readers
Geographical breakdown
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|---|---|---|
| Unknown | 47 | 100% |
Demographic breakdown
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|---|---|---|
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| Student > Ph. D. Student | 6 | 13% |
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|---|---|---|
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| Unknown | 16 | 34% |