Chapter title |
Advances in Agrobacterium-mediated Maize Transformation
|
---|---|
Chapter number | 3 |
Book title |
Maize
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7315-6_3 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7314-9, 978-1-4939-7315-6
|
Authors |
Heng Zhong, Sivamani Elumalai, Samson Nalapalli, Lee Richbourg, Anna Prairie, David Bradley, Shujie Dong, Xiujuan Jenny Su, Weining Gu, Tim Strebe, Liang Shi, Qiudeng Que, Zhong, Heng, Elumalai, Sivamani, Nalapalli, Samson, Richbourg, Lee, Prairie, Anna, Bradley, David, Dong, Shujie, Su, Xiujuan Jenny, Gu, Weining, Strebe, Tim, Shi, Liang, Que, Qiudeng |
Abstract |
One of the major limitations of maize transformation is the isolation of a large number of immature embryos using the time-consuming manual extraction method. In this article, we describe a novel bulk embryo extraction method for fast isolation of a large number of embryos suitable for both biolistic- and Agrobacterium-mediated transformation. Optimal gene delivery and tissue culture conditions are also described for achieving high efficiency in Agrobacterium-mediated maize transformation using phosphomannose isomerase (PMI) as a selectable marker. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 11 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 4 | 36% |
Student > Postgraduate | 1 | 9% |
Researcher | 1 | 9% |
Unknown | 5 | 45% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 3 | 27% |
Biochemistry, Genetics and Molecular Biology | 2 | 18% |
Medicine and Dentistry | 1 | 9% |
Unknown | 5 | 45% |