| Chapter title |
Virus-Induced Gene Silencing in Maize with a Foxtail mosaic virus Vector
|
|---|---|
| Chapter number | 7 |
| Book title |
Maize
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7315-6_7 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7314-9, 978-1-4939-7315-6
|
| Authors |
Yu Mei, Steven A. Whitham |
| Abstract |
Virus-induced gene silencing (VIGS) is a powerful technology for rapidly and transiently knocking down the expression of plant genes to study their functions. A VIGS vector for maize derived from Foxtail mosaic virus (FoMV), a positive-sense single-stranded RNA virus, was recently developed. A cloning site created near the 3' end of the FoMV genome enables insertion of 200-400 nucleotide fragments of maize genes targeted for silencing. The recombinant FoMV clones are inoculated into leaves of maize seedlings by biolistic particle delivery, and silencing is typically observed within 2 weeks after inoculation. This chapter provides a protocol for constructing FoMV VIGS clones and inoculating them into maize seedlings. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 21 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 6 | 29% |
| Student > Doctoral Student | 3 | 14% |
| Researcher | 2 | 10% |
| Lecturer > Senior Lecturer | 1 | 5% |
| Student > Bachelor | 1 | 5% |
| Other | 4 | 19% |
| Unknown | 4 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 10 | 48% |
| Biochemistry, Genetics and Molecular Biology | 5 | 24% |
| Unspecified | 1 | 5% |
| Chemistry | 1 | 5% |
| Unknown | 4 | 19% |