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Chapter title |
Single Molecule FRET Analysis of DNA Binding Proteins
|
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Chapter number | 12 |
Book title |
Single Molecule Analysis
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Published in |
Methods in molecular biology, January 2018
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DOI | 10.1007/978-1-4939-7271-5_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7270-8, 978-1-4939-7271-5
|
Authors |
Kathy R. Chaurasiya, Remus T. Dame |
Abstract |
The complex binding dynamics between DNA and proteins are often obscured by ensemble averaging effects in conventional biochemical experiments. Single-molecule fluorescence methods are powerful tools to investigate DNA-protein interaction dynamics in real time. In this chapter, we focus on using single-molecule Förster Resonance Energy Transfer (smFRET) to probe the binding dynamics of individual proteins on single DNA molecules. We provide a detailed discussion of total internal reflection fluorescence (TIRF) instrument design, nucleic acid labeling with fluorophores, flow cell surface passivation, and data analysis methods. |
Mendeley readers
The data shown below were compiled from readership statistics for 23 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 23 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 8 | 35% |
Student > Master | 7 | 30% |
Researcher | 4 | 17% |
Student > Bachelor | 1 | 4% |
Unspecified | 1 | 4% |
Other | 1 | 4% |
Unknown | 1 | 4% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 7 | 30% |
Agricultural and Biological Sciences | 5 | 22% |
Physics and Astronomy | 3 | 13% |
Engineering | 2 | 9% |
Chemistry | 2 | 9% |
Other | 3 | 13% |
Unknown | 1 | 4% |