| Chapter title |
RNA Unzipping and Force Measurements with a Dual Optical Trap
|
|---|---|
| Chapter number | 2 |
| Book title |
Single Molecule Analysis
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7271-5_2 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7270-8, 978-1-4939-7271-5
|
| Authors |
Laurent Geffroy, Pierre Mangeol, Thierry Bizebard, Ulrich Bockelmann, Geffroy, Laurent, Mangeol, Pierre, Bizebard, Thierry, Bockelmann, Ulrich |
| Abstract |
In order to mechanically unfold a single RNA molecule, an RNA/DNA hybrid construction is prepared which allows specific attachment to two micrometer-sized beads. A dual-beam optical trap thus holding the construct in solution captures the beads separately. Unfolding of a molecule is obtained by increasing the distance between the traps, one trap being slowly moved while the other is held fixed. Force is measured to sub-piconewton precision by back focal plane interferometry of the bead in the fixed trap. The experiment allows us to measure structure and base-sequence-dependent force signals. In this chapter, important technical aspects of this type of single-molecule force measurements are considered. |
Mendeley readers
Geographical breakdown
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|---|---|---|
| Unknown | 4 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 1 | 25% |
| Student > Postgraduate | 1 | 25% |
| Student > Master | 1 | 25% |
| Unknown | 1 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 2 | 50% |
| Physics and Astronomy | 1 | 25% |
| Unknown | 1 | 25% |