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Mendeley readers
| Chapter title |
Protein Tethering for Folding Studies
|
|---|---|
| Chapter number | 3 |
| Book title |
Single Molecule Analysis
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7271-5_3 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7270-8, 978-1-4939-7271-5
|
| Authors |
Fatemeh Moayed, Roeland J. van Wijk, David P. Minde, Sander J. Tans |
| Abstract |
Optical tweezers allow the detection of unfolding and refolding transitions in individual proteins, and how interacting molecules such as chaperones affect these transitions. Typical methods that tether individual proteins are based on cysteine chemistry, which is less suitable for proteins with essential cysteines. Here we describe a cysteine-independent tethering protocol that can be performed in situ. |
Mendeley readers
The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 4 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 1 | 25% |
| Student > Postgraduate | 1 | 25% |
| Student > Master | 1 | 25% |
| Unknown | 1 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 2 | 50% |
| Nursing and Health Professions | 1 | 25% |
| Unknown | 1 | 25% |