Chapter title |
Analysis of Proteins Enriched in Rice Gamete
|
---|---|
Chapter number | 20 |
Book title |
Plant Germline Development
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7286-9_20 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7285-2, 978-1-4939-7286-9
|
Authors |
Takashi Okamoto |
Abstract |
In angiosperms, female gamete differentiation, fertilization, and subsequent zygotic development occur in embryo sacs deeply embedded in the ovaries. Despite their importance in plant reproduction and development, how the egg cell is specialized, fuses with the sperm cell, and converts into an active zygote for early embryogenesis remains unclear. Proteins enriched in each cell type are thought to reflect the biological function of the cells, since the composition of cellular proteins generally differs depending on cell type. Therefore, proteins enriched in gametes, egg and sperm cells, should have a role in reproductive and/or developmental processes, such as gamete differentiation, gamete fusion, egg activation, and early zygotic development. In this chapter, a method for identification of proteins enriched in rice gametes is first explained, and then functional analyses for these proteins using rice and Arabidopsis lines with mutations in genes encoding the putative gamete-enriched proteins are also described. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 8 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Unspecified | 1 | 13% |
Student > Ph. D. Student | 1 | 13% |
Student > Bachelor | 1 | 13% |
Researcher | 1 | 13% |
Student > Master | 1 | 13% |
Other | 0 | 0% |
Unknown | 3 | 38% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 3 | 38% |
Unspecified | 1 | 13% |
Agricultural and Biological Sciences | 1 | 13% |
Unknown | 3 | 38% |