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Plant Germline Development

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Cover of 'Plant Germline Development'

Table of Contents

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    Book Overview
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    Chapter 1 An Introduction to Male Germline Development
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    Chapter 2 Apomixis: Engineering the Ability to Harness Hybrid Vigor in Crop Plants
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    Chapter 3 3D Imaging of Whole-Mount Ovules at Cellular Resolution to Study Female Germline Development in Rice
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    Chapter 4 Live-Cell Imaging of F-Actin Dynamics During Fertilization in Arabidopsis thaliana
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    Chapter 5 Development and Observation of Mature Megagametophyte Cell-Specific Fluorescent Markers
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    Chapter 6 Analysis of Fluorescent Reporter Activity in the Male Germline During Pollen Development by Confocal Microscopy
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    Chapter 7 In Vivo Ploidy Determination of Arabidopsis thaliana Male and Female Gametophytes
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    Chapter 8 Staining and Clearing of Arabidopsis Reproductive Tissue for Imaging of Fluorescent Proteins
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    Chapter 9 Live-Cell Imaging of Auxin and Cytokinin Signaling in Maize Female Gametophytes
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    Chapter 10 Imaging Ca2+ Dynamics in Wild-Type and NADPH Oxidase-Deficient Mutant Pollen Tubes with Yellow Cameleon and Confocal Laser Scanning Microscopy
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    Chapter 11 Immunolocalization of AGPs and Pectins in Quercus suber Gametophytic Structures
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    Chapter 12 Optimization of Cell Spreading and Image Quality for the Study of Chromosomes in Plant Tissues
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    Chapter 13 Whole Mount RNA-FISH on Ovules and Developing Seeds
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    Chapter 14 Analysis of Peroxisome Biogenesis in Pollen by Confocal Microscopy and Transmission Electron Microscopy
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    Chapter 15 Transmission Electron Microscopy (TEM) to Study Histology of Pollen and Pollen Tubes
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    Chapter 16 Isolation of Arabidopsis Pollen, Sperm Cells, and Vegetative Nuclei by Fluorescence-Activated Cell Sorting (FACS)
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    Chapter 17 Isolation of Rice Sperm Cells for Transcriptional Profiling
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    Chapter 18 Manual Isolation of Living Cells from the Arabidopsis thaliana Female Gametophyte by Micromanipulation
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    Chapter 19 Isolation and Detection of Small RNAs from Pollen
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    Chapter 20 Analysis of Proteins Enriched in Rice Gamete
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    Chapter 21 Phosphoprotein Enrichment from Tobacco Mature Pollen Crude Protein Extract
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    Chapter 22 Identification of Cis-Regulatory Modules that Function in the Male Germline of Flowering Plants
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    Chapter 23 RNA-Seq Data Analysis: From Raw Data Quality Control to Differential Expression Analysis
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    Chapter 24 RNA-Seq Data Analysis Protocol: Combining In-House and Publicly Available Data
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    Chapter 25 Impedance Flow Cytometry as a Tool to Analyze Microspore and Pollen Quality
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    Chapter 26 Agrobacterium-Mediated Sorghum Transformation
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    Chapter 27 Use of the Cas9 Orthologs from Streptococcus thermophilus and Staphylococcus aureus for Non-Homologous End-Joining Mediated Site-Specific Mutagenesis in Arabidopsis thaliana
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    Chapter 28 Transgenic Reproductive Cell Ablation
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    Chapter 29 In Vivo Reporters for Protein Half-Life
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    Chapter 30 Conditional Modulation of Biological Processes by Low-Temperature Degrons
Attention for Chapter 14: Analysis of Peroxisome Biogenesis in Pollen by Confocal Microscopy and Transmission Electron Microscopy
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Chapter title
Analysis of Peroxisome Biogenesis in Pollen by Confocal Microscopy and Transmission Electron Microscopy
Chapter number 14
Book title
Plant Germline Development
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-7286-9_14
Pubmed ID
Book ISBNs
978-1-4939-7285-2, 978-1-4939-7286-9
Authors

Peng-Fei Jia, Hong-Ju Li, Wei-Cai Yang

Abstract

Peroxisome is an essential single-membrane bound organelle in most eukaryotic cells and functions in diverse cellular processes. De novo formation, division, and turnover of peroxisomes contribute to its biogenesis, morphology, and population regulation. In plants, peroxisome plays multiple roles, including metabolism, development, and stress response. Defective peroxisome biogenesis and development retard plant growth, adaption, and reproduction. Through tracing the subcellular localization of fluorescent reporter tagged matrix protein of peroxisome, fluorescence microscopy is a reliable and fast way to detect peroxisome biogenesis. Further fine-structural observation of peroxisome by TEM enables researchers to observe the detailed ultrastructure of its morphology and spatial contact with other organelles. Pollen grain is a specialized structure where two small sperm cells are enclosed in the cytoplasm of a large vegetative cell. Two features make pollen grain a good system to study peroxisome biogenesis: indispensable requirement of peroxisome for germination on the stigma and homogeneity. Here, we describe the methods of studying peroxisome biogenesis in Arabidopsis pollen grains by fluorescent live-imaging with confocal laser scanning microscopy (CLSM) and by DAB-staining based transmission electron microscopy (TEM).

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 7 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 43%
Unspecified 1 14%
Student > Bachelor 1 14%
Student > Master 1 14%
Unknown 1 14%
Readers by discipline Count As %
Agricultural and Biological Sciences 4 57%
Biochemistry, Genetics and Molecular Biology 1 14%
Unspecified 1 14%
Unknown 1 14%