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Plant Germline Development

Overview of attention for book
Cover of 'Plant Germline Development'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 An Introduction to Male Germline Development
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    Chapter 2 Apomixis: Engineering the Ability to Harness Hybrid Vigor in Crop Plants
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    Chapter 3 3D Imaging of Whole-Mount Ovules at Cellular Resolution to Study Female Germline Development in Rice
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    Chapter 4 Live-Cell Imaging of F-Actin Dynamics During Fertilization in Arabidopsis thaliana
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    Chapter 5 Development and Observation of Mature Megagametophyte Cell-Specific Fluorescent Markers
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    Chapter 6 Analysis of Fluorescent Reporter Activity in the Male Germline During Pollen Development by Confocal Microscopy
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    Chapter 7 In Vivo Ploidy Determination of Arabidopsis thaliana Male and Female Gametophytes
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    Chapter 8 Staining and Clearing of Arabidopsis Reproductive Tissue for Imaging of Fluorescent Proteins
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    Chapter 9 Live-Cell Imaging of Auxin and Cytokinin Signaling in Maize Female Gametophytes
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    Chapter 10 Imaging Ca2+ Dynamics in Wild-Type and NADPH Oxidase-Deficient Mutant Pollen Tubes with Yellow Cameleon and Confocal Laser Scanning Microscopy
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    Chapter 11 Immunolocalization of AGPs and Pectins in Quercus suber Gametophytic Structures
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    Chapter 12 Optimization of Cell Spreading and Image Quality for the Study of Chromosomes in Plant Tissues
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    Chapter 13 Whole Mount RNA-FISH on Ovules and Developing Seeds
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    Chapter 14 Analysis of Peroxisome Biogenesis in Pollen by Confocal Microscopy and Transmission Electron Microscopy
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    Chapter 15 Transmission Electron Microscopy (TEM) to Study Histology of Pollen and Pollen Tubes
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    Chapter 16 Isolation of Arabidopsis Pollen, Sperm Cells, and Vegetative Nuclei by Fluorescence-Activated Cell Sorting (FACS)
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    Chapter 17 Isolation of Rice Sperm Cells for Transcriptional Profiling
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    Chapter 18 Manual Isolation of Living Cells from the Arabidopsis thaliana Female Gametophyte by Micromanipulation
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    Chapter 19 Isolation and Detection of Small RNAs from Pollen
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    Chapter 20 Analysis of Proteins Enriched in Rice Gamete
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    Chapter 21 Phosphoprotein Enrichment from Tobacco Mature Pollen Crude Protein Extract
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    Chapter 22 Identification of Cis-Regulatory Modules that Function in the Male Germline of Flowering Plants
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    Chapter 23 RNA-Seq Data Analysis: From Raw Data Quality Control to Differential Expression Analysis
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    Chapter 24 RNA-Seq Data Analysis Protocol: Combining In-House and Publicly Available Data
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    Chapter 25 Impedance Flow Cytometry as a Tool to Analyze Microspore and Pollen Quality
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    Chapter 26 Agrobacterium-Mediated Sorghum Transformation
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    Chapter 27 Use of the Cas9 Orthologs from Streptococcus thermophilus and Staphylococcus aureus for Non-Homologous End-Joining Mediated Site-Specific Mutagenesis in Arabidopsis thaliana
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    Chapter 28 Transgenic Reproductive Cell Ablation
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    Chapter 29 In Vivo Reporters for Protein Half-Life
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    Chapter 30 Conditional Modulation of Biological Processes by Low-Temperature Degrons
Attention for Chapter 15: Transmission Electron Microscopy (TEM) to Study Histology of Pollen and Pollen Tubes
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Chapter title
Transmission Electron Microscopy (TEM) to Study Histology of Pollen and Pollen Tubes
Chapter number 15
Book title
Plant Germline Development
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-7286-9_15
Pubmed ID
Book ISBNs
978-1-4939-7285-2, 978-1-4939-7286-9

Peng-Fei Jia, Hong-Ju Li, Wei-Cai Yang


Here, we describe methods of transmission electron microscopy (TEM) based on conventional chemical fixation and high-pressure freezing (HPF) and freeze-substitution (FS) to examine the ultrastructure of Arabidopsis pollen grains and pollen tubes. Compared to other plant samples, such as root or leaf, pollen grains have thick pollen coat and cell wall which limit the permeation of fixative. Thus, it is difficult to obtain high-quality ultrastructural images of pollen. Moreover, pollen tube is very soft and the traditional procedure is too harsh to get an intact pollen tube sample. Up to now, there is no available mature protocol for TEM sample preparation of Arabidopsis pollen tube. Here, we describe the details and step-by-step procedures of chemical fixation, HPF, FS, and resin-embedding protocols for Arabidopsis pollen and pollen tube. In addition, we also provide a method on how to get longitudinal sections of pollen tubes.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 8 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 2 25%
Student > Bachelor 1 13%
Researcher 1 13%
Other 1 13%
Unknown 3 38%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 50%
Agricultural and Biological Sciences 2 25%
Physics and Astronomy 1 13%
Unknown 1 13%

Attention Score in Context

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